Review





Similar Products

96
Vector Laboratories brightvision anti rabbit ap
Brightvision Anti Rabbit Ap, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/brightvision anti rabbit ap/product/Vector Laboratories
Average 96 stars, based on 1 article reviews
brightvision anti rabbit ap - by Bioz Stars, 2026-04
96/100 stars
  Buy from Supplier

94
MedChemExpress ecl plus substrate
Ecl Plus Substrate, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ecl plus substrate/product/MedChemExpress
Average 94 stars, based on 1 article reviews
ecl plus substrate - by Bioz Stars, 2026-04
94/100 stars
  Buy from Supplier

96
Vector Laboratories vector red alkaline phosphatase (red ap) substrate kit
Vector Red Alkaline Phosphatase (Red Ap) Substrate Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vector red alkaline phosphatase (red ap) substrate kit/product/Vector Laboratories
Average 96 stars, based on 1 article reviews
vector red alkaline phosphatase (red ap) substrate kit - by Bioz Stars, 2026-04
96/100 stars
  Buy from Supplier

96
Vector Laboratories vector red
Vector Red, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vector red/product/Vector Laboratories
Average 96 stars, based on 1 article reviews
vector red - by Bioz Stars, 2026-04
96/100 stars
  Buy from Supplier

96
Vector Laboratories vector blue
Vector Blue, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vector blue/product/Vector Laboratories
Average 96 stars, based on 1 article reviews
vector blue - by Bioz Stars, 2026-04
96/100 stars
  Buy from Supplier

96
Vector Laboratories vector blue alkaline phosphatase (blue ap) substrate kit
Vector Blue Alkaline Phosphatase (Blue Ap) Substrate Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vector blue alkaline phosphatase (blue ap) substrate kit/product/Vector Laboratories
Average 96 stars, based on 1 article reviews
vector blue alkaline phosphatase (blue ap) substrate kit - by Bioz Stars, 2026-04
96/100 stars
  Buy from Supplier

90
Millipore immobilon western chemiluminescent hrp substrate
Immobilon Western Chemiluminescent Hrp Substrate, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/immobilon western chemiluminescent hrp substrate/product/Millipore
Average 90 stars, based on 1 article reviews
immobilon western chemiluminescent hrp substrate - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

96
Vector Laboratories novared
Novared, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/novared/product/Vector Laboratories
Average 96 stars, based on 1 article reviews
novared - by Bioz Stars, 2026-04
96/100 stars
  Buy from Supplier

94
New England Biolabs cell nonpermeable snap surface alexa fluor 488 substrate
Biochemical and live-cell imaging characterization of the effects of insulin on INSR. (A) Internalized to total INSR ratio was quantified using surface biotinylation in undifferentiated C2C12 myoblasts. Cells were incubated in PBS (no insulin) or serum-free DMEM containing 0, 0.2, 2, or 20 nM insulin for 15 minutes. The ratios are normalized to the 0 nM group of each gel ( P > .05 when not specified). (B) Western blot showing SNAP-tagged INSR expressed from lentiviral vector in comparison to wild-type INSR in C2C12 myoblasts. (C, D) Representative images of INSR-A-SNAP-labeled using an Alexa <t>Fluor</t> <t>488</t> cell nonpermeable dye in 0, 0.2, or 20 nM insulin conditions in live undifferentiated C2C12 myoblasts from (C) 0 to 15 minutes or (D) after 20 minutes using a spinning disk confocal microscope. Time-lapse images of INSR vesicle interactions starting from the selected subregions (white squares) of snapshots are shown in the insets ( n = 3 cells).
Cell Nonpermeable Snap Surface Alexa Fluor 488 Substrate, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cell nonpermeable snap surface alexa fluor 488 substrate/product/New England Biolabs
Average 94 stars, based on 1 article reviews
cell nonpermeable snap surface alexa fluor 488 substrate - by Bioz Stars, 2026-04
94/100 stars
  Buy from Supplier

Image Search Results


Biochemical and live-cell imaging characterization of the effects of insulin on INSR. (A) Internalized to total INSR ratio was quantified using surface biotinylation in undifferentiated C2C12 myoblasts. Cells were incubated in PBS (no insulin) or serum-free DMEM containing 0, 0.2, 2, or 20 nM insulin for 15 minutes. The ratios are normalized to the 0 nM group of each gel ( P > .05 when not specified). (B) Western blot showing SNAP-tagged INSR expressed from lentiviral vector in comparison to wild-type INSR in C2C12 myoblasts. (C, D) Representative images of INSR-A-SNAP-labeled using an Alexa Fluor 488 cell nonpermeable dye in 0, 0.2, or 20 nM insulin conditions in live undifferentiated C2C12 myoblasts from (C) 0 to 15 minutes or (D) after 20 minutes using a spinning disk confocal microscope. Time-lapse images of INSR vesicle interactions starting from the selected subregions (white squares) of snapshots are shown in the insets ( n = 3 cells).

Journal: Journal of the Endocrine Society

Article Title: Insulin receptor trafficking and interactions in muscle cells

doi: 10.1210/jendso/bvag020

Figure Lengend Snippet: Biochemical and live-cell imaging characterization of the effects of insulin on INSR. (A) Internalized to total INSR ratio was quantified using surface biotinylation in undifferentiated C2C12 myoblasts. Cells were incubated in PBS (no insulin) or serum-free DMEM containing 0, 0.2, 2, or 20 nM insulin for 15 minutes. The ratios are normalized to the 0 nM group of each gel ( P > .05 when not specified). (B) Western blot showing SNAP-tagged INSR expressed from lentiviral vector in comparison to wild-type INSR in C2C12 myoblasts. (C, D) Representative images of INSR-A-SNAP-labeled using an Alexa Fluor 488 cell nonpermeable dye in 0, 0.2, or 20 nM insulin conditions in live undifferentiated C2C12 myoblasts from (C) 0 to 15 minutes or (D) after 20 minutes using a spinning disk confocal microscope. Time-lapse images of INSR vesicle interactions starting from the selected subregions (white squares) of snapshots are shown in the insets ( n = 3 cells).

Article Snippet: Cell nonpermeable SNAP-Surface Alexa Fluor 488 substrate (Cat. #S9129S, New England Biolabs) was dissolved and diluted to 5 μM concentration in serum-free medium containing 0.5% bovine serum albumin according to the manufacturer's instruction.

Techniques: Live Cell Imaging, Incubation, Western Blot, Plasmid Preparation, Comparison, Labeling, Microscopy